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Biology of ageing: principles, challenges and perspectives Suresh I. Rattan Living systems owe their survival and health to a series of complex biochemical pathways of maintenance and repair.

These defense systems create the homeodynamic space of an individual, which is characterized by stress tolerance, molecular damage control and continuous remodeling. Ageing, age-related diseases and eventual death are the consequences of a progressive shrinkage of the homeodynamic space, due to the failure of maintenance and repair. Whereas longevity assurance genes do affect the essential lifespan of a species, there are no ageing-specific gerontogenes to cause ageing and to limit the lifespan of an individual.

The challenge of preventing, memorial hermann pierdere în greutate de zahăr or treating age-related chronic diseases and other health problems requires abandoning the traditional one-target, one-shot biomedical approach.

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Wholistic methods incorporating lifestyle-based hormetic interventions, including food, physical activity and mental engagement, appear to be potentially more successful in maintaining health and in extending healthspan and longevity.

Corresponding author: rattan mbg. Molecular signatures of cardiac stem cells Cristian Bogdan Iancu, Daniela Iancu, Irina Rentea, Sorin Hostiuc, Dan Dermengiu, Mugurel Constantin Rusu The discovery of cardiac stem ratu slimming review jelly CSCs able to renew the pool of cardiomyocyte raised the question of how these cells can be recognized and directed towards cardiac reconstruction after severe ischemic injury. The functional studies demonstrated that the differentiation of adult cardiac stem cells reproduce the stages observed in the embryonic development.

Each stage is characterized by a complex molecular signature, which can be used for identification and molecular targeting.

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Three major markers have been used to isolate CSCs: c-kit, Sca-1, and Isl1 and different progenitor populations have been described: side-population SPcardiosphere-derived, epicardial-derived. Combinations between the main three markers and other transcription factors, cell surface proteins and regulatory RNAs may delimit ratu slimming review jelly further the cardiac precursors.

Accumulation of data leads to the idea that a single, yet unidentified unique cardiac stem cell is at the origin of those observed variants. In this review, we intended to summarize the actual knowledge about the main molecular markers of cardiac stem cells. Needle-based confocal laser endomicroscopy in pancreatic cystic tumors assessment Daniela Stefanescu, Stephen P. Pereira, Margaret Keane, Adrian Saftoiu Pancreatic cystic tumors PCT are relatively common findings in general population due to the widespread use of cross-sectional imaging.

PCT can be benign, with premalignant potential or malignant, a different management being applied for each type: benign cysts are usually referred for follow-up based on imagingwhile premalignant or malignant lesions should be surgically resected. The aim of this review is to describe the latest imaging technique that could be used for PCT diagnosis and to establish its clinical impact. Endoscopic ultrasound EUS is generally used to evaluate a pancreatic mass and to identify its characteristics.

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It offers a good visualization of the lesion. When combined with fine needle aspiration and cystic fluid analysis, the diagnosis potential is increased, although its accuracy for differentiating benign and malign tumors remains modest. EUS-guided needle-based confocal laser endomicroscopy nCLE is a new imaging technique that uses a miniprobe thin enough to be passed through a 19G needle.

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It provides in vivo images of the pancreas at ratu slimming review jelly cellular level, offering the possibility to assess any changes that might have occurred. Regarding the safety, more studies are needed. EBV is implicated in the etiology of a significant number of neoplasms, which acquire particular features in terms ratu slimming review jelly course and prognosis.

Incidence rates are much higher in children. The role of EBV in patient response to oncological treatment remains controversial. Materials and Methods: Diagnostic tissues from 56 nasopharyngeal carcinoma patients and 32 nasopharyngitis patients were evaluated retrospectively by immunohistochemical analysis for the expression of CSF-1R.

Results: Significant differences of CSF-1R expression exists between nasopharyngeal carcinoma patients and nasopharyngitis patients p Corresponding author: Ximing Xu, Archiater; e-mail: whuxxm yahoo.

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The impact of two-socket preservation approaches on the soft and hard tissue healing: a short-term study in dogs Cosmin Vasile Cioban, Raluca Zaganescu, Alexandra Roman, Stefan Adrian Petrutiu, Florin Ioan Beteg, Marius Mihai Muste, Darian Rusu, Carmen Mihaela Mihu The present study was designed in the context of the uncertain circumstances related to the best therapeutic option for ridge preservation.

The research aimed to investigate the quality of early healing processes developed in the former sockets preserved with a collagen matrix alone or associated with a bone substitute in comparison with naturally-healed sockets, using an animal model previously validated.

In both quadrants of the mandible of two dogs, the distal sockets of the second and fourth premolars served as experimental sites.

Two sockets healed naturally, three sockets were preserved with the collagen matrix and three sockets were preserved with the collagen matrix plus a bone substitute. After one month of healing, the samples were harvested and histologically processed. The soft tissue covering the preserved ridges displayed an obviously thicker epithelial layer containing mostly areas of parakeratinized epithelium alternating with keratinized ones in comparison with naturally-healed sockets.

In the apical third of the sockets, a mature bone structure was recorded for all three types of post-extraction approaches. While in naturally-healed and collagen matrix-preserved specimens the central third of the sockets contained bone with a mature aspect, in collagen matrix plus bone substitute-preserved sockets an immature appearance was observed.

In the external third of the sockets, only in matrix-preserved alveoli a well-developed cancellous bone was present. The bovine bone substitute seemed to delay hard tissue development. The use of the collagen matrix could be a clinical option to preserve ratu slimming review jelly ridges especially when an improvement in soft tissue quality is desired.

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Ischemic preconditioning and inflammatory response syndrome after reperfusion injury: an experimental model in diabetic rats Bianca Liana Grigorescu, Anca Meda Georgescu, Adrian-Dan Cioc, Raluca-Stefania Fodor, Ovidiu Simion Cotoi, Pal Fodor, Sanda Maria Copotoiu, Leonard Azamfirei Quantification of local ischemia and inflammatory response syndrome correlated with histological changes associated with ischemia-reperfusion injury IRI after revascularization techniques.

We included 12 adult male Wistar rats, aged eight weeks that were randomly divided into two groups. After eight weeks, the rats were subject to ischemic preconditioning for 10 minutes at three regular intervals. Twenty-four proteine pentru slabit si definire post-preconditioning, both groups were subject to ischemia for 20 minutes, followed by 30 minutes of reperfusion.

Oxygen extraction was higher in Group 1, the arterio-venous CO2 gradient was higher in the control group, but not significant. The lactate production was higher in Group 1. The muscle samples from the control group displayed significant rhabdomyolysis, damage to the nucleus, while the preconditioned group showed almost normal morphological characteristics.

The lungs and kidneys were most damaged in the control group, with damage expressed as thickened alveolar septa, neutrophil infiltrates, eosinophilic precipitates in the proximal convolute tubule. Ischemic preconditioning significantly attenuates the ischemic reperfusion injury. The assessment of immunohistochemical profile of endometriosis implants, a practical method to appreciate the aggressiveness and recurrence risk of endometriosis Elvira Bratila, Cornel Petre Bratila, Diana-Elena Comandasu, Vasilica Bausic, Camelia Teodora Vladescu, Claudia Pot să mănânc sănătos și să pierd greutatea, Costin Berceanu, Monica Mihaela Cirstoiu, George Mitroi, Ruxandra Stanculescu Endometriosis represents a chronic female genital tract disease characterized by implants outside the endometrial cavity, leading to alteration of ratu slimming review jelly anatomy and having as result chronic pelvic pain and infertility.

Aim: From the molecular perspective, the aim of studying endometriosis is identifying a cause and a consequence, that lead to the appearance and perpetual arising of new implants. The description of the immunohistochemical IHC profile of ectopic endometrium could represent a new element in the pathogenesis ratu slimming review jelly endometriosis and also a practical method to appreciate the aggressiveness and possibility of recurrence of the disease.

The study consisting of histopathological and immunohistochemical Ratu slimming review jelly analysis of the tissues excised included 14 patients, operated from June to Decemberto which was confirmed the presumptive diagnosis of endometriosis, based on anamnesis, clinical examination and ultrasound appearance.

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We identified the expression of estrogen and progesterone receptors, whose presence in the ectopic endometrium guides the medical hormone postoperative treatment.

We also identified the expression of a cellular ratu slimming review jelly marker - Ki, and inhibition marker of cellular apoptosis - Bcl-2, in order to characterize the aggressiveness of endometriosis implantations and a stromal marker CD Although there are plenty of medical and surgical therapeutic methods available, the treatment of endometriosis must be individualized for every patient taking into consideration the IHC analysis.

Consolidation of surgical treatment by prescription of a medical long-term treatment is indispensable, because endometriosis is a chronic relapsing disease. A computerized morphometric algorithm was designed and applied in both red-green-blue RGB and hue-lightness-saturation HLS color spaces, allowing the automated measurement of areas for the following morphological characteristics of the renal corpuscles RCs : glomerulus, Bowman space, cells, mesangial matrix and glomerular basement membranes, and capillaries.

Consequently, we assume that ratu slimming review jelly morphometrical characterization of RCs histological components could be used as an additional criterion not ratu slimming review jelly in the diagnosis of MPGNs, but also in the stratification of evolution and prognosis of patients diagnosed with type I and II MNGN, respectively.

Materials and Methods: Digital images of prostate adenocarcinoma labeled following both Gleason and Srigley systems were acquired with x20 objective from two serial sections, from the section stained using Gomori technique for SFC assessment and another corresponding from the section immunomarked with anti-CD34 antibody for assessment of VD.

Results: SFC mean values had an ascending trend in both solid and necrotizing groups. VD mean values had an ascending trend in solid group but a descending trend in necrotizing group towards Gleason 5A pattern. The two stromal elements had different behaviors both individually and in their correlation that seem to be related with their interaction with different tumor cellular populations. Conclusions: Our results could plead for the hypothesis that the different subtypes of tumor architecture represent steps of a continuous process from well-differentiated status to poorly or undifferentiated status but who is accomplished by ratu slimming review jelly different tumor cells populations with different distinct behavior in their relationship with the stromal microenvironment.

Lipopolysaccharide LPS administration is the most often used approach to model the consequences of bacterial sepsis. We created an endotoxemia rat model, simulating sepsis-related lung injury, in order to quantify the time and dose dependent severity lesions induced by the administration of lipopolysaccharide. At six hours, the animals included in the groups with higher doses of LPS developed thrombocytopenia, elevated lactate levels, and liver and renal injury in a dose and time dependent manner.

The severity of hypoxemia at six hours correlated with the increasing doses of LPS, with a slight improvement at 24 hours. Lung injury scores became more severe with increased dose and time of exposure to LPS without reaching the level of hyaline membranes formation. We also demonstrated translocation of a protein from the airspaces into plasma RAGE - receptor for advanced glycation end products.

Induction of sepsis using LPS is a known experimental model, but LPS treatment in ratu slimming review jelly does not cause the severe endothelial and epithelial injury that occurs in humans with acute respiratory distress syndrome ARDS.

In our study, the clinical, laboratory and histopathological findings confirmed sepsis and the damage of the alveolar-capillary membrane in a dose-dependent manner.

Orthodontic tooth movement following analgesic treatment with Aspirin and Algocalmin. Rp pierderea în greutate experimental study Cristian Doru Olteanu, Alin Serbanescu, Adina Bianca Bosca, Carmen Mihaela Mihu The objective of this study was the tracing of the effect of Aspirin and Algocalmin, two of the most frequently used analgesics after the application of orthodontic appliances, on the dental displacements, starting from the hypothesis according to whom any substance that inhibits prostaglandins production will have as result the inhibition of the osteoclasts activity and of the orthodontic tooth movements implicit.

Twenty-four male Wistar rats, separated into three groups were used.

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Group I ratu slimming review jelly included eight rats in which the orthodontic device was applied, without a subsequent administration of any analgesic; Group II - eight rats in which after the device application Aspirin was administrated; and Group III - eight animals in which Algocalmin was administrated.

A histological study was completed in order to establish the size of bone areola. Average mesial displacement of the first left inferior maxillary molar 28 days after applying the orthodontic device was of 3.

The average displacement in the group in which Aspirin was administrated was 0. In the group treated with Algocalmin, the dental displacement was of 0. Histological examination revealed the presence of large sizes bone areola in control group micro-mmore reduced in the group treated with Aspirin 74 micro-mand intermediate in that treated with Algocalmin micro-m. Treatments with Aspirin and Algocalmin in experimental groups, immediately after the orthodontic device application, induced a decreased dental displacement rate.

An experimental study PDF The aim of the study was to assess especially the morphological differences between gastric carcinomas revealing one morphological feature and those including two morphological features.

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Materials and Methods: Two groups of patients were selected: Group 1, including 43 patients with tumors revealing only one architectural pattern, and Ratu slimming review jelly 2, including 16 patients with two architectural patterns within the tumor. In addition to gender and age, the main morphological parameters were: lesion location and macroscopic appearance on the surgical excision sample and microscopic appearance of the surgical excision sample, assessed for architectural pattern, secretory properties and prognosis based on histological features, degree of tumor extension and the degree of tumor aggressiveness, using a wide range of histological and immunohistochemical stainings.

All data were compared between the two groups using statistical tests. Results and Discussion: Significant differences were observed between the values and distributions of morphological parameters in the two groups and were discussed comparatively.

Conclusions: Tumors with two dominant histological aspects present simultaneously are a reality that cannot be argued but whose morphological and biological profile needs to be completed and validated.